The Opentrons OT-2 is well suited for creating some of the Origami Assays pooling designs. Below I provide descriptions, deck layouts, and python code to run the XS2, M, and XL designs.
Note on decoding: The OT-2 designs here are reformatted to a 24 well format instead of the 96 well format used in the main Origami Assays layout. The 24 well format was chosen here because it matches the form factor for pooling primary patient samples coming from 2-3 ml tubes.
The Origami Assay's decoder has the option to enter positive pools by pool ID instead of by well ID. For example, if you go to the decode page for the M design you will see an option to "input result by pool index" circled in red below:
All subsequent decoding is the same, just the way to specify which pooled sample wells were positive is different.
All of the python scripts are patterne after the OT-2 documented pooling example and use the following custom labware file for 24 well plates:
XS2
The XS2 design is the S design repeated twice so as to fully fill a single 24 well plate.
To build the XL design on the OT-2 requires four stages. At each stage, four source plates are replaced and the pipette tips are replaced, but the two target plates remain.
