Research

---

Multiplex assay design is an area of active research in the scientific community and within Origami Assays. In particular, we are interested in exploring, documenting, and comparing the performance of different experimental design strategies when faced with realistic error rates and larger prevalance rates.

---

1) Overview

• Pathogen screening
• A small example: Testing 6 employees
• Other applications

---

2) Monoplex

• Background
• 270 Sample example

---

3) Nonadaptive multiplex

• Background
• 270 Sample example
• Prevalance estimation using pooling alone
• Matrix design and theory

---

4) Semi-adaptive

• Background
• 270 Sample example
• 1122 Sample example
• Robusness and fragility to assay error rates

---

5) Fully adaptive

• Background
• 270 Sample example

---

Physical Assay Design

Liquid Handling

• Liquid handling speed and timing
• Harnessing the 8- and 12-multichannel pipette
• Paper liquid handling templates

Sample Capture

• Mask based COVID19 assays