q-DATO: qPCR Diagnostic Assay Throughput Optimization
The goal of q-DATO is to help you get the most data out of your quantitative PCR diagnostic assays. Many common diagnostic assays use multiple probes to measure a single target, and run each sample in monoplex to maximize sensitivity. However, if greater assay throughput is required, it may be possible to use fewer or pooled probes, or pool samples together. These asasy changes can produce nearly the same data quality while using many fewer assays.
q-DATO allows you to load and analyze
your own qPCR data to predict how various assay changes would affect the diagnostic sensitivity.